Background

The nELISA Technology

Three strategies put together to bring about step-function change.

Colocalization for True Multiplexing

Using DNA as a flexible, programmable linker to completely overcome cross-reactivity.

Traditional Multiplexing

Traditional Multiplexing

Mixing antibodies in traditional multiplexed ELISAs leads to spurious cross-reactivity.

Proximity-based Multiplexing

Proximity-based Multiplexing

Using complementary strands with PCR or NGS to minimize cross-reactivity, after it has occured.

Colocalization by nELISA

Colocalization (1)Colocalization (2)

Using universal DNA structures to pre-assemble antibody pairs on their cognate beads prevents cross-reactivity from occurring in the first place. That way, the signal is never impacted by the level of multiplexing, achieving True Multiplexing.

Next-Level Fluorescent Barcoding

The power of combinatorial spectral multiplexing unlocked using nano-manufacturing and physical models.

4

dyes

4 dyes graph

>3,000

spectral barcodes

4 dyes graph

High-throughput, simple workflow

>10,000 samples per week using a surprisingly simple workflow - all using commercially available instrumentation.

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Background

Learn about nELISA targets

Towards proteome-wide interrogation, we’ve started with the inflammatory secretome. See what targets we’ve on-boarded to-date, what targets are coming up next, and how you can add your own on the Targets page.