nELISA: True Multiplexing at Scale

What if simple refinements to the age-old ELISA could have profound impacts on its scalability?

nELISA Technology

nucleobase Enabled Localized Immunoassay with Spectral Addressing

Colocalization for True Multiplexing

Using flexible DNA linkers to pre-assemble antibody pairs to completely side step cross-reactivity.

Traditional Multiplexing

Mixing antibodies in traditional multiplexed ELISAs leads to spurious cross-reactivity.

Proximity-based Multiplexing

Using complementary DNA strands with PCR or NGS to minimize cross-reactivity, after it has occurred.

Colocalization by nELISA

Using universal DNA structures to pre-assemble antibody pairs on their cognate beads prevents cross-reactivity from occurring in the first place. That way, the signal is never impacted by the level of multiplexing, achieving True Multiplexing.

Next-Level Fluorescent Barcoding

The power of combinatorial spectral multiplexing unlocked using nano-manufacturing and physical models.

4

Dyes

>3,000

Spectral Barcodes

nELISA by the numbers

~0.1 pg/mL​

High-sensitivity​
assays​

<10% CV​

Intra-lot reproducibility

3-6 log​

Per assay
dynamic range​

99.99%​

Specificity​

High Throughput​

Tens of thousands of samples per week​

Cost Efficient​

Low cost per sample​

The nELISA: A Massively Multiplexed & Miniaturized Immunoassay Platform for High-Throughput Protein Profiling

The nELISA brings a quantum leap to protein profiling by enabling the scaling of content without the need to compromise on throughput or cost. By enabling scientists to measure more proteins in more samples for less, Nomic is supporting new discoveries and accelerating the development of new drugs and diagnostics.

Nomic MELISA technical document mockup with data visualization pages
Blue gradient background with soft white bubbles on right side
Protein Target Library

Expertly curated to balance the critical must-haves, biological breadth, and real-world utility.

End-to-End Services

Explore the most extensive protein profiling services.